Human donor eyes were collected, characterized, and prepared for immunohistochemistry by J.D. Messinger DC (Curcio lab since 2007). All were preserved ≤6 hours from death by immersion in 4% paraformaldehyde, 0.1 M phosphate buffer after removal of cornea/ lens/ iris (stored in 1% paraformaldehyde). Ex vivo fundus imaging includes OCT, color, near-infrared reflectance, and autofluorescence. Cryosections (2/slide) are 12 µm thick, ≥ 20 mm long, parallel to horizontal meridian, through fovea and perifovea, collected on SuperFrost slides (positively charged, borosilicate glass, 1 mm thick, subbed with poly-L-lysine). Sample size based on power analysis of RPE dysmorphia (Simon et al, IOVS 2022; 63:5).